Pooling Samples
Technique
以前體檢隊慣用的手法，常被批評為偷雞摸狗的方法。如今美國FDA說(June 16, 2020) 無症狀者的COVID-19大量篩檢可以這樣做，還稱之為是一種技術。可降低大量篩檢所投入的資源，並更快發出篩檢報告。
新冠病毒的RT-PCR因有基因擴增的程序，假陰性或許可降低，但仍難避免。那其他檢驗項目若採用此技術，造成假陰性的機率會是多少?
FDA提醒採用pooling samples technique，必須先通過方法確認(validation)。醫檢師又有新課題可以探討了。
FDA
statement:
In order to preserve testing resources, many developers are interested in performing their testing using a technique of “pooling” samples. This technique allows a lab to mix several samples together in a “batch” or pooled sample and then test the pooled sample with a diagnostic test......................However, because samples are diluted, which could result in less viral genetic material available to detect, there is a greater likelihood of false negative results, particularly if not properly validated.
This method works well when there is a low prevalence of cases, meaning more negative results are expected.
In order to preserve testing resources, many developers are interested in performing their testing using a technique of “pooling” samples. This technique allows a lab to mix several samples together in a “batch” or pooled sample and then test the pooled sample with a diagnostic test......................However, because samples are diluted, which could result in less viral genetic material available to detect, there is a greater likelihood of false negative results, particularly if not properly validated.
This method works well when there is a low prevalence of cases, meaning more negative results are expected.
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